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KMID : 1145120160140010006
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Asian Journal of Beauty and Cosmetology
2016 Volume.14 No. 1 p.6 ~ p.17
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Potential of Fisetin as a Nutri-cosmetics Material through Evaluating Anti-oxidant and Anti-adipogenic Activities
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Shon Myung-Soo
Kim Ryeong-Hyeon
Song Ji-Hye
Kwon Oh-Jun
Lee Ah-Reum
Kim Hae-Ok
Roh Seong-Soo
Kim Gyo-Nam
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Abstract
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Purpose: Although several physiological properties of fisetin have been reported, the potential of fisetin as a nutri-cosmetics material has been not considered. Therefore, anti-oxidant and anti-adipogenic effects in 3T3-L1 cells of fisetin were evaluated and its potential as a nutri-cosmetic ingredient was discussed.
Methods: To test anti-oxidant activity of fisetin, ABTS radical scavenging assay was used and the cytotoxicity of fisetin in 3T3-L1 preadipocytes was evaluated by EZ-cytox assay. The inhibitory effect of fisetin against 3T3-L1 adipogenesis was evaluated in various stage of adipogenesis such as pre-adipogenic (Day -2~0), early (Day 0~2), intermediate (Day 2~4), and terminal stage (Day 4~6) by oil red o (ORO) staining. The mRNA expression levels of transcriptional factors were performed by RT-PCR analysis.
Results: We observed that the scavenging activity of fisetin against ABTS radicals was stronger than that of ascorbic acid, a well-known soluble anti-oxidant. Fisetin treatment resulted in the decreased triglyceride content of 3T3-L1 cells in a dose-dependent manner. Lipid accumulation of 3T3-L1 adipocytes was significantly inhibited by fisetin treatment by 54.61%, 61.74%, 56.82%, and 26.67%, respectively when compared to adipocytes control. The anti-adipogenic effect of fisetin was strongly limited in the pre-adipogenic, early, and intermediate stages of 3T3-L1 adipogenesis. In addition, the fisetin treatment down-regulated mRNA expression levels of C/EBPβ, leptin, and adiponectin in mature 3T3-L1 adipocytes.
Conclusion: These results suggested that fisetin has the potential as a nutri-cosmetic material which possess anti-oxidant and anti-adipogenic activities.
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KEYWORD
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Fisetin, Nutri-cosmetics, Anti-oxidant, Adipogenesis, Obesity
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